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🔗 Genetics 🔗 Molecular Biology/Molecular and Cell Biology

In genetic engineering, a gene gun or biolistic particle delivery system is a device used to deliver exogenous DNA (transgenes), RNA, or protein to cells. By coating particles of a heavy metal with a gene of interest and firing these micro-projectiles into cells using mechanical force, an integration of desired genetic information can be induced into cells. The technique involved with such micro-projectile delivery of DNA is often referred to as biolistics.

This device is able to transform almost any type of cell and is not limited to the transformation of the nucleus; it can also transform organelles, including plastids and mitochondria.

🔗 Electronic music 🔗 Music/Music genres

Lowercase is an extreme form of ambientminimalism where very quiet, usually unheard sounds are amplified to extreme levels. Minimal artist Steve Roden popularized the movement with an album entitled Forms of Paper, in which he made recordings of himself handling paper in various ways. These recordings were commissioned by the Hollywood branch of the Los Angeles Public Library.

🔗 Russia 🔗 Literature 🔗 Russia/performing arts in Russia 🔗 Russia/language and literature of Russia

Chekhov's gun (Russian: Чеховское ружьё) is a dramatic principle that states that every element in a story must be necessary, and irrelevant elements should be removed. Elements should not appear to make "false promises" by never coming into play. The statement is recorded in letters by Anton Chekhov several times, with some variation:

• "Remove everything that has no relevance to the story. If you say in the first chapter that there is a rifle hanging on the wall, in the second or third chapter it absolutely must go off. If it's not going to be fired, it shouldn't be hanging there."
• "One must never place a loaded rifle on the stage if it isn't going to go off. It's wrong to make promises you don't mean to keep." Chekhov, letter to Aleksandr Semenovich Lazarev (pseudonym of A. S. Gruzinsky), 1 November 1889. Here the "gun" is a monologue that Chekhov deemed superfluous and unrelated to the rest of the play.
• "If in the first act you have hung a pistol on the wall, then in the following one it should be fired. Otherwise don't put it there." From Gurlyand's Reminiscences of A. P. Chekhov, in Teatr i iskusstvo 1904, No. 28, 11 July, p. 521.

Ernest Hemingway mocked the interpretation given by English instructors to the principle. He gives in his essay "The Art of the Short Story" an example of two characters that are introduced and then never again mentioned in his short story "Fifty Grand". Hemingway valued inconsequential details, but conceded that readers will inevitably seek symbolism and significance in these inconsequential details.

🔗 Chemicals 🔗 Occupational Safety and Health 🔗 Food and drink 🔗 Rocks and minerals 🔗 Chemicals/Chemicals worklist

Borax, also known as sodium borate, sodium tetraborate, or disodium tetraborate, is an important boron compound, a mineral, and a salt of boric acid. Powdered borax is white, consisting of soft colorless crystals that dissolve in water. A number of closely related minerals or chemical compounds that differ in their crystal water content are referred to as borax, and the word is usually used to refer to the octahydrate. Commercially sold borax is partially dehydrated.

Borax is a component of many detergents, cosmetics, and enamel glazes. It is used to make buffer solutions in biochemistry, as a fire retardant, as an anti-fungal compound, in the manufacture of fiberglass, as a flux in metallurgy, neutron-capture shields for radioactive sources, a texturing agent in cooking, as a cross-linking agent in Slime, as an alkali in photographic developers, as a precursor for other boron compounds, and along with its inverse, boric acid, is useful as an insecticide.

In artisanal gold mining, borax is sometimes used as part of a process (as a flux) meant to eliminate the need for toxic mercury in the gold extraction process, although it cannot directly replace mercury. Borax was reportedly used by gold miners in parts of the Philippines in the 1900s.

Borax was first discovered in dry lake beds in Tibet and was imported via the Silk Road to the Arabian Peninsula in the 8th century AD. Borax first came into common use in the late 19th century when Francis Marion Smith's Pacific Coast Borax Company began to market and popularize a large variety of applications under the 20 Mule Team Borax trademark, named for the method by which borax was originally hauled out of the California and Nevada deserts.

🔗 United States/U.S. Government 🔗 United States 🔗 Human rights 🔗 Military history 🔗 Military history/North American military history 🔗 Military history/United States military history 🔗 Medicine 🔗 Skepticism 🔗 Psychology 🔗 Military history/Intelligence 🔗 Alternative Views 🔗 Psychoactive and Recreational Drugs 🔗 Drug Policy 🔗 Science Policy

Project MKUltra (or MK-Ultra), also called the CIA mind control program, is the code name given to a program of experiments on human subjects that were designed and undertaken by the U.S. Central Intelligence Agency, some of which were illegal. Experiments on humans were intended to identify and develop drugs and procedures to be used in interrogations in order to weaken the individual and force confessions through mind control. The project was organized through the Office of Scientific Intelligence of the CIA and coordinated with the United States Army Biological Warfare Laboratories. Code names for drug-related experiments were Project Bluebird and Project Artichoke.

The operation was officially sanctioned in 1953, reduced in scope in 1964 and further curtailed in 1967. It was officially halted in 1973. The program engaged in many illegal activities, including the use of U.S. and Canadian citizens as its unwitting test subjects, which led to controversy regarding its legitimacy. MKUltra used numerous methods to manipulate its subjects' mental states and brain functions. Techniques included the covert administration of high doses of psychoactive drugs (especially LSD) and other chemicals, electroshocks, hypnosis, sensory deprivation, isolation, verbal and sexual abuse, as well as other forms of torture.

The scope of Project MKUltra was broad, with research undertaken at more than 80 institutions, including colleges and universities, hospitals, prisons, and pharmaceutical companies. The CIA operated using front organizations, although sometimes top officials at these institutions were aware of the CIA's involvement.

Project MKUltra was first brought to public attention in 1975 by the Church Committee of the United States Congress and Gerald Ford's United States President's Commission on CIA activities within the United States (also known as the Rockefeller Commission).

Investigative efforts were hampered by CIA Director Richard Helms' order that all MKUltra files be destroyed in 1973; the Church Committee and Rockefeller Commission investigations relied on the sworn testimony of direct participants and on the relatively small number of documents that survived Helms's destruction order. In 1977, a Freedom of Information Act request uncovered a cache of 20,000 documents relating to project MKUltra which led to Senate hearings later that year. Some surviving information regarding MKUltra was declassified in July 2001. In December 2018, declassified documents included a letter to an unidentified doctor discussing work on six dogs made to run, turn and stop via remote control and brain implants.

🔗 California 🔗 California/San Francisco Bay Area 🔗 Internet

The "PayPal Mafia" is a group of former PayPal employees and founders who have since founded and developed additional technology companies such as Tesla Motors, LinkedIn, Palantir Technologies, SpaceX, YouTube, Yelp, and Yammer. Most of the members attended Stanford University or University of Illinois at Urbana–Champaign at some point in their studies.

🔗 Automobiles 🔗 Japan 🔗 Japan/Car

Kei car (or keijidōsha, kanji: 軽自動車, "light automobile", pronounced [keːdʑidoːɕa]), known variously outside Japan as Japanese city car, ultramini, or Japanese microcar, is the Japanese vehicle category for the smallest highway-legal passenger cars. Similar Japanese categories exist for microvans, and kei trucks. These vehicles are most often the Japanese equivalent of the EU A-segment (city cars).

The kei-car category was created by the Japanese government in 1949, and the regulations have been revised several times since. These regulations specify a maximum vehicle size, engine capacity, and power output, so that owners may enjoy both tax and insurance benefits. In most rural areas they are also exempted from the requirement to certify that adequate parking is available for the vehicle.

Kei cars have become very successful in Japan, consisting of over one-third of domestic new-car sales in fiscal 2016, despite dropping from a record 40% market share in 2013, after the government increased the kei-car tax by 50% in 2014. In 2018, seven of the 10 top-selling models were kei cars, including the top four, all boxy passenger vans: Honda N-Box, Suzuki Spacia, Nissan Dayz, and Daihatsu Tanto. Isuzu is the only manufacturer that has never offered a kei-sized vehicle for either private ownership or commercial trucks and microvans.

In export markets, though, the genre is generally too specialized and too small for most models to be profitable. Notable exceptions exist, though, for instance the Suzuki Alto and Jimny models, which were exported consistently from around 1980. Kei cars are not only popular with the elderly, but they are also popular with youths because of their affordability.

Nearly all kei cars have been designed and manufactured in Japan, but a version of the French-made Smart was briefly imported and officially classified as a kei car, and since then, the British Caterham 7 160 has also received such classification.

🔗 Biography 🔗 United Kingdom 🔗 Ships

The rescue of Roger Mallinson and Roger Chapman occurred between 29 August and 1 September 1973 after their Vickers Oceanics small submersible Pisces III was trapped on the seabed at a depth of 1,575 ft (480 m), 150 mi (240 km) off Ireland in the Celtic Sea. The 76-hour multinational rescue effort resulted in the deepest sub rescue in history.

🔗 United States 🔗 Biography 🔗 United States/Massachusetts 🔗 Mammals 🔗 Zoo

Hoover (c. 1971 – July 25, 1985) was a harbor seal who was able to imitate basic human speech.

🔗 United States 🔗 Finance & Investment 🔗 Economics 🔗 Business

The Panic of 1907 – also known as the 1907 Bankers' Panic or Knickerbocker Crisis – was a financial crisis that took place in the United States over a three-week period starting in mid-October, when the New York Stock Exchange fell almost 50% from its peak the previous year. Panic occurred, as this was during a time of economic recession, and there were numerous runs on banks and trust companies. The 1907 panic eventually spread throughout the nation when many state and local banks and businesses entered bankruptcy. Primary causes of the run included a retraction of market liquidity by a number of New York City banks and a loss of confidence among depositors, exacerbated by unregulated side bets at bucket shops.

The panic was triggered by the failed attempt in October 1907 to corner the market on stock of the United Copper Company. When this bid failed, banks that had lent money to the cornering scheme suffered runs that later spread to affiliated banks and trusts, leading a week later to the downfall of the Knickerbocker Trust Company—New York City's third-largest trust. The collapse of the Knickerbocker spread fear throughout the city's trusts as regional banks withdrew reserves from New York City banks. Panic extended across the nation as vast numbers of people withdrew deposits from their regional banks. It is the 9th largest decline in U.S. stock market history.

The panic might have deepened if not for the intervention of financier J. P. Morgan, who pledged large sums of his own money, and convinced other New York bankers to do the same, to shore up the banking system. This highlighted the impotence of the nation's Independent Treasury system, which managed the nation's money supply yet was unable to inject liquidity back into the market. By November, the financial contagion had largely ended, only to be replaced by a further crisis. This was due to the heavy borrowing of a large brokerage firm that used the stock of Tennessee Coal, Iron and Railroad Company (TC&I) as collateral. Collapse of TC&I's stock price was averted by an emergency takeover by Morgan's U.S. Steel Corporation—a move approved by anti-monopolist president Theodore Roosevelt. The following year, Senator Nelson W. Aldrich, a leading Republican, established and chaired a commission to investigate the crisis and propose future solutions, leading to the creation of the Federal Reserve System.